Acute Toxicity and Respiratory Responses in Freshwater Fish, Labeo Rohita exposed to An Agrochemical Indoxacarb

N. Bantu, Z. Hagos, C. Krishna, G. krishnan, A. Abaynew, V. Rathnamma, R. babu

Abstract


Abstract: In the present study, an attempt has been made to analyze the toxicity of the Indoxacarb on the freshwater fish Labeo rohita. The toxicity tests were conducted to choose the mortality range from 10% to 90% for 24, 48, 72 and 96 h in static system. Finney’s probit analysis, (Finney, 1971) was followed to calculate the LC50 values. Experimental fish were exposed to different concentrations of Indoxacarb for different hours, percent mortality was recorded. The 96 h LC50 value of toxicant to the fish were found to be 0.0521 mg/L. Throughout the experimental period, the fish showed severe respiratory distress and rapid opercular movements leading to the higher amount of toxicant uptake, increased secretion of mucus higher ventilation volume, decrease in oxygen uptake efficiency, labored breathing and engulfing of air through the mouth  Behavioral patterns were observed in during exposure period, test organism showed normal behavior in control group but jerky movements, hyper secretion of mucus, opening and closing of mouth for gasping, losing scales, hyperactivity were observed experimental group. The results suggest that indoxacarb is considered as hazardous pesticide in common carp with 96 hrs LC50 value.  Also, the mortality rate increased with increase in the concentration of pesticide. All the studies mentioned above indicate a considerable effect of insecticides on oxygen consumption in different species of fish in lethal as well as sub lethal concentrations.


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Elizabeth D. Hilborn and Val R. Beasley.One Health and Cyanobacteria in Freshwater Systems: Animal Illnesses and Deaths Are Sentinel Events for Human Health Risks Toxins 2015, 7: 1374-1395.

Palaniappan, P.L.R.M and karthikeyan. Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel, 2009 J.Environ.sci, 21:229-236.

Toni., Candida, Vania Lucia loro., Adriana santi, Charlene cavalheiro de Menezes, Roberta cattaneo, Barbara estevao clasen, Renato zanella, Exposure to tebuconazol in rice field and laboratory conditions induces oxidative stress in carp(Cyprinus carpio).Comparative Biochemistry and physiology, Part C, 2011, 153:128-132.

Zubair Ahmad, Toxicity bioassay and effects of sublethal exposure of malathion on biochemical composition and hematological parameters of Clarias gariepinus. African Journal of Biotechnology, 2012, 11(34):8578-8585.

Nagaraju Bantu and Rathnamma vakita,. Effect of Dimethoate on mortality and Biochemical changes of Freshwater fish Labeo rohita (Hamilton). Journal of Biology and today’s world, 2013, 2(10):456-470.

Nagaraju Bantu, Venkata rathnamma Vakita, Somaiah Karra. Effect of Chlorantraniliprole on Biochemical and Certain Biomarkers in Various Tissues of Freshwater Fish Labeo rohita (Hamilton). Environment and Ecology Research, 2013, 1: 205 – 215.

Doudoroff P, Anderson B. O, Burdick G. E, Galtsoff P. S, Hart W. B; Parick R; Strong E. R, Surber E. W; Vanhorn W. M, Bioassay methods for the evaluation of acute toxicity of industrial wastes to fish, Sewage Industry Wastes, 1951, 23:1380.

APHA. American Public Health Association. Standard methods for examination of water including bottom sediments and sludges. Standard Methods, 2005, 19th ed., p. 874.

Shivakumar, H.B. Effect of dimethoate on toxicity and oxygen consumption of the freshwater teleost, Cyprinus carpio, J Ecotox Environ Monit, 2008, 18(40): 319-324.

Saroja, K .Oxygen consumption in relation to body size and temperature in the earthworm, Megascholex mauritii when submerged under water. Proc. Indian. Acad. Sod. (Anim. Sci.), 1959, 49: 183-193.

Smyser, B.P. Batch equilibrium study of DPX-JW062 (a racemic mixture of DPX-KN128 and IN-KN-127) and IN-JT333. DuPont Report No. AMR 3489-95, Rev. 1. E. I. du Pont de Nemours and Company, 2002, Wilmington, DE.

U. S. EPA. Pesticide Fact Sheet: Indoxacarb. Litster S. Mathematical modeling of fuel cells for portable devices. M.Sc. Thesis, University of Victoria, 2005.

Veeraiah K., Rao S. Changes in biochemical parameters of freshwater fish Labeo rohita exposed to lethal and sub-lethal concentrations of indoxacarb. International Journal of Bioassays, 2013, 2: 1282-1387.

Ali Taheri Mirghaed and Melika Ghelichpour .Determination of lethal concentrations of indoxacarb in fingerling Cyprinus carpio at two temperatures.International Journal of Aquatic Biology, 2015, 3(5):339-345.

Muthukumaravel, K., Sukumaran, M and Sathick, O, Studies on the acute toxicity of pesticides on the freshwater fish Labeo rohita. J. Pure Appl. Zool. 2013, 1(2): 185-192.

Patil V. K. and David M .Behavior and respiratory dysfunction as an index of Malathion toxicity in the freshwater fish, Labeo rohita (Hamilton). Turk. J. Fish. Aquat. Sci. 2008, 8: 233-237.

Johnson, W.W and Finely, M.T. Handbook of acute toxicity of chemicals to fish and aquatic invertebrates. U.S. Fish wilddl. Serv. Resource. Publication unit, U.S. fish and wildlife service, Washington, Dc, 20240., 1980, ISBN-13: 978-1125461129

Koprucu S.S., Koprucu K., Ural M.S., Ispir, U and Pala M . Acute toxicity of organophosphorus pesticide Diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis L.). Pesticide Biochemistry and Physiology, 2006, 86: 99-105.

Marigoudar S R, Ahmed R N and David M. Impact of Cypermethrin on behavioral responses in the fresh water teleost, Labeo rohita (Hamilton), World Journal of Zoology, 2009, 4 (1): 19-23.

Parithabhanu A, Pesticide toxicity and behavioral responses in the fish Orechromis mossambicus. International Journal of Fisheries and Aquaculture Sciences, 2013, 3(2): 161-164.

Kalavathy, K., Siva Kumar, A.A. and Chandran, R. Toxic effects of the pesticide dimethoate on the fish, Sarotherodon mossambicus. 2001, J. Ecol. Res. Bio, 2: 27-32.

Katja, S., Georg, B.O.S., Stephan, P. and Christian, E.W.S. Impact of PCB mixture (Aroclor 1254) and TBT and a mixture of both on swimming behavior, body growth and enzymatic biotransformation activities (GST) of young carp, Cyprinus carpio. Aquatic Toxicology, 2005, 71: 49-59.

Bhat BA, Bhat IA, Vishwakarma S, Verma A, Saxena G. A Comparative Study on the Toxicity of a Synthetic Pesticide, Dichlorvos and a Neem based Pesticide, Neem-On to Labeo rohita (Hamilton), Curr world Environ, 2012, 7(1):157-161.

Finney D. J 1971. Probit Analysis, 3rd edn. Cambridge University Press, Cambridge, 1971,20.

Magare, S.R. and Patil, H.T. Effect of pesticides on oxygen consumption, red blood cell count and metabolites of a fish, Puntius ticto. Environ. Ecology, 2000, 18: 891-894.

Nagaraju.Bantu and Venkatarathnamma. Gas liquid chromatography-flame ionization detector (GLC-FID) residue analysis of carbamate pesticide in freshwater fish Labeo rohita, Toxicology Research, 2014,3:177-183.

Ram nayan Singh. Acute toxicity of an organophosphate, dimethoate to an air breathing fish, Colisa fasciatus (Bl. & Schn.), Indian J.Sci.Res, 2013,4(1): 97-100.

Subramani lavanya,Mathan Ramesh,Chokkalingam kavitha, Annamalai Malarvizhi .Hematological, biochemical and ion regulatory responses of Indian major carp Catla catla during chronic sub lethal exposure to inorganic arsenic, Chemosphere, 2011,82:977-85

Welsh JH, Smith, RI, 1953. Laboratory exercises in invertebrate’s physiology. Burgess Publish. Co., Minnepolis, p. 126.


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