Étude de la production de bioéthanol biocarburant à partir de sous-produits agricoles : Effet de l’aération
Abstract
Abstract: Among bioenergetic processes, fermentation of effluents from agricultural by-products is a promising alternative for energy recovery of discharges. The biofuels produced can be exploited as additives for conventional fuels. Moreover, they can be produced from agricultural by-products rich in carbohydrates. The aim of our work is to produce bioethanol from a mixture of agricultural feedstocks, that is: the by-products of grapes and dates, by using the yeast Saccharomyces Cerevisiae in a cylindrical bioreactor with a capacity of 5 L. The effect of aeration on bioethanol yield has been studied. Different parameters have been monitored such as: bioethanol concentration, total sugar, ammoniacal nitrogen, pH, yeast viability, etc. It was found a high bioethanol concentration for flow rate 50 mL/min. In such case, the bioethanol concentration reached it was about 160 g/L which is higher in comparison to that obtained for a flow rate of 100 mL / min or in anaerobic which is 38 g/L and 112 g/L, respectively.
Résumé: Parmi les procédés bioénergétiques, la fermentation des effluents issus de sous-produits agricoles est une alternative prometteuse à la valorisation énergétique des rejets. Les biocarburants produits peuvent être exploités comme additifs aux carburants conventionnels. De plus, ils peuvent être produits à partir de sous-produits agricoles riches en glucides. Le but de notre travail consiste à produire du bioéthanol à partir de matières premières agricoles à savoir : les sous-produits de dattes et de raisins, et ce en présence de la levure Saccharomyces cerevisiae dans un bioréacteur cylindrique de capacité 5 L. L'effet de l'aération sur le rendement en bioéthanol (g/g de sucre) a été étudié. Différents paramètres ont été suivis tels que : la concentration en bioéthanol, la croissance de la levure, les sucres totaux, l’azote ammoniacal et le pH. Une production élevée en bioéthanol a été trouvée pour un débit d’air de 50 mL/min. Dans ce cas, la concentration en bioéthanol atteinte est de 160 g/L qui est élevée par comparaison à celle obtenue pour un débit de 100 mL/min ou en anaérobie qui est de 38 g/L et 112 g/L, respectivement.
Full Text:
PDFReferences
Riess, J. Intensification de la brique « fermentation alcoolique » de substrats betterave (et autre substrats) pour la production d’éthanol , Thèse de Doctorat, Université Toulouse (2012)177.
Pandiyan, K.; Singh, A.; Singh, S.; Saxena, A.K.; Nain, L. Technological interventions for utilization of crop residues and weedy biomass for 2second generation bio-ethanol production, Renewable Energy, 132 (2019)723-741.
Ntaikou, I.; Menis N., Alexandropoulou, M.; Antonopoulou, G.; Lyberatos, G. Valorization of kitchen biowaste for ethanol production via simultaneous saccharification and fermentation using co-cultures of the yeasts Saccharomyces cerevisiae and Pichia stipitis, Bioresource Technology, 263 (2018) 75-83.
Costa,-Nogueira, C.C.; Padilh,a C.E.A.; Jesus, A.A.; Souza, D.F.S.; Assis, C.F.; Junior, F.C..S.; Santo, E.S. Pressurized pretreatment and simultaneous saccharification and fermentation with in situ detoxification to increase bioethanol production from green coconut fibers, Industrial Crops and Products, 130 (2019) 259-266.
Park, J.; Han, T.; Yarish, C.; Kim, J.K. Microalgae and Alcohol, Microalgae in Health and Disease Prevention (2018) 227-234.
Arshad, M.; Hussain, T.; Iqbal, M.; Abbas, M. Enhanced ethanol production at commercial scale from molasses using high gravity technology by mutant Saccharomyces Cerevisiae, Brazilian Journal of Microbiology, 48 (3) (2017) 403-409.
Ballerini, D. Les biocarburants : Etat des lieux, perspectives et enjeux du développement, IFP publication, Edition Technip, Paris (2006)
Lareo C.; Ferrari M.D., Guigou, M.; Fajardo, L.; Larnaudie, V.; Ramírez, M.B.; Martínez-Garreiro, J. Evaluation of sweet potato for fuel bioethanol production: hydrolysis and fermentation. Springer Plus, 2 (1) (2013) 493.
Joannis-Cassan, C.; Riess, J.; Jolibert, F.; Taillandier, P. Optimization of very high gravity fermentation process for ethanol production from industrial sugar beet syrup, Biomass and Bioenergy, 70 (2014) 165-173.
Cekmecelioglu, D.; Uncu, ON.Kinetic modeling of enzymatic hydrolysis of pretreated kitchen wastes for enhancing bioethanol production, Waste Manage, 33 (3) (2013) 735–9.
Mansouri, A. ; Rihani, R ; Laoufi, N.A. Etude de la production d’éthanol biocarburant à partir de rejets agricoles, Second International Conference on Mechanics ICM, Constantine, Algérie, (2015) 25/26 Novembre.
Mansouri, A.; Rihani, R.; Laoufi, A. N.; Özkan, M. Production of bioethanol from a mixture of agricultural feed stocks: Biofuels characterization, Fuel, 185 (2016) 612-621.
Jones R.P., Measures of yeast death and deactivation and their meaning: Part I, Process Biochem., 22 (1987)118-128.
Bapat, P.; Nandy, S. K.; Wangikar, P.; Venkatesh, K.V. Quantification of metabolically active biomass using Methylene Blue dye reduction test (MBRT): Measurement of CFU in about 200 s, J. Microbiol. Methods, 65 (2006) 107-116.
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F., Colorimetric method for determination of sugars and related substances, Analytical Chemistry, 28 (3) (1956): 350-356.
Herbert D., Phipps P. J., Strange R. E., Chemical analysis of microbial cells, Methods in microbiology, 5 (Part B) (1971) 209-344.
Alfenor, S.; Cameleyre, X.; Bandadi,s L.; Bideaux, C.; Uribelarrea, J.L.; Goma, G.; Molina-Jouve, C.; Guillouet, S.E. Aeration strategy: A need for veryhigh ethanol performance in Saccharomyces cerevisiae fed-batch process., Appl. Microbiol. Biotechnol, 63 (2004)(5) 537-42.
Isla, M.; Comelli, R.N.; Seluy,. L.G. Wastewater from the soft drinks industry as a source for bioethanol production, Argentina, Bioresource technology, 136 (2013) 140-7.
Bely, M.; Sablayrolles, J.M.; Barre, P. Automatic detection of assimilable nitrogen deficiencies during alcoholic fermentation in oenological conditions Journal of fermentation and bioengineering, 70 (4) (1990) 246-252.
Refbacks
- There are currently no refbacks.