Characterization by spectrometric methods of chitin produced from white shrimp shells of Parapenaeus longirostris byLactobacillus helveticus cultivated on glucose or date waste

W. Arbia, L. Arbia, L. Adour, A. Amrane, A. Benhadji, H. Lounici

Abstract


Abstract: Chitin recovery by lactic acid fermentation was considered. It was previously shown that the use of glucose led to 61% of demineralization and 42% of deproteinization; while and unlike glucose, the inoculation of L. helveticus in date waste (200 g L-1 reducing sugars) led to a simultaneous demineralization and deproteinization of shrimp shells, 83 and 61 %, respectively. The purity of end-products were characterized by infrared (FTIR), X-ray diffraction and X-ray fluorescence.

Analysis of the chemical composition of fermentation end-product by X-ray fluorescence showed that residual minerals are in trace except CaO and P2O5. The infrared spectra and the X-ray diffractograms of both samples compared to those of pure chitin showed a strong similarity. FTIR of fermented shells showed the appearance of characteristic peaks of α-chitin and the disappearance of calcite peaks. In addition and especially for the shells fermented using L. helveticus cultivated on date waste, a similarity with those of pure chitin should be noted. These results confirmed the efficiency of biological chitin recovery, and that a synthetic carbon source (glucose) can be replaced by a natural one, date waste, allowing a valorization of this latter. The relevance of subsequent works to improve yields of demineralization and deproteinization was therefore confirmed.

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Oh, Y-S.; Shih, I-L.;Tzeng, Y-M.; Wang, S-L. Protease produced by Pseudomonas aeruginosa K-187 and its application in the deproteinization of shrimp and crab shell wastes. Enzyme andMicrobial Technology 27 (2000) 3- 10.

Noishiki, Y.;Takami, H.;Nishiyama, Y.; Wada, M.; Okada, S.; Kuga, S. Alkali-Induced Conversion of β-Chitin to α-Chitin.Biomacromoles4 (2003) 896- 899.

Goodrich, D.J.; Winter, W.α-chitin from shrimp shells nanocrystals Prepared and their SpecificArea measurement. Biomacromoles8 (2007) 252 - 257.

Cho, Y.I.; No, H.K.; Meyers, S.P. Physicochemical characteristics and functional properties of various commercial chitin and chitosan products. Journal of AgriculturalFood Chemistry 46 (1998) 3839-3843.

Ifuku, S.;Nogi, M.; Abe, K.; Yoshioka, M.; Morimoto, M.;Saimoto, H.; Yano, H. Preparation of chitin nanofibers with a uniform width as α-chitin from crab shells. Biomacromoles 10 (2009) 1584–1588.

Zhang, Y.; Xue, C.; Xue, Y.; Gao, R.; Zhang, X. Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction. Carbohydrate Research 340 (2005) 1914–1917.

Dahiya, N.; Tewari, R.; Hoondal, G.S. Biotechnological aspects of chitinolytic enzymes: a review. Applied Microbiology and Biotechnology71 (2006) 773- 782.

Franca, E.F.; Lins, R.D.; Freitas, L.C.; Straatsma, T. P. Characterization of Chitin and Chitosan Molecular Structure in Aqueous Solution. Journal of theoretical Chemistry and Computation. 4 (2008) 2141–2149.

Yasutomo, N.; Hiroko, T.; Yoshiharu, N.; Masahisa, W.; Shigeru, O.; Shigenori, K. Alkali-Induced Conversion of α-Chitin to β-Chitin. Biomacromoles 4 (2003) 896- 899.

Rinaudo, M.; Chitin and chitosan: Properties and applications. Progress in Polymer Science31 (2006) 603–632.

Xu, Y.; Gallert, C.; Winter, J. Chitin purification from shrimp wastes by microbial deproteination and decalcification. AppliedMicrobiology and Biotechnology 79 (2008) 687–697.

Yen, M-T.;Yang, J-H.;Mau, J-L.Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymer75 (2009) 15–21.

Shahidi, F.;Arachchi, J. K. V.;Jeon, Y-J.Food applications of chitin and chitosans. Trends in Food Science and Technology 10 (1999) 37–51.

Valdez-Peña, A. U.; Espinoza-Perez, J. D.; Sandoval-Fabian, G.C.;Balagurusamy, N.; Hernandez-Rivera, A.; De-la-Garza, I.M.; Contreras-Esquivel, J.C. Screening of industrial enzymes for deproteinization of shrimp head for chitin recovery.Food Science and Biotechnology 19 (2010) 553- 557.

Jung, W.J.;Kuk, J.H.; Kim, K.Y.; Park, R.D. Demineralization of red crab shell waste by lactic acid Fermentation. ApplliedMicrobiology and Biotechnology 67 (2005) 851–854.

Adour, L.; Arbia, W.; Amrane, A.; Mameri, N. Combined use of waste materials – recovery of chitin from shrimpshells by lactic acid fermentation supplemented with date juice waste or glucose. Journal of Chemistry Technology and Biotechnology 83 (2008) 1664–1669.

Fagbenro, O. Preparation, properties and preservation of lactic acid fermentedshrimp heads. Food Research International29 (1996) 595- 599.

Shirai, K.; Guerrero, I.; Huerta S.; Saucedo, G.; Casillo A.; Obdulia, G.R.; Hall M.G. Effect of initial glucose and inoculation level of lactic acid bacteria in shrimp waste ensilation. Enzyme and Microbial Technology 28 (2001) 446- 452.

Bautista, J.;Jover, M.;Guttierrez, J.F.;Corpas, R.;Cremades, O.;Fontiveros, E.; Iglesias, F.; Vega, J. Preparation of crayfish chitin by in situ lactic acid production. Process Biochemistry 37(2001) 229- 234.

Zakaria, Z.; Hall, G. M.; Shama, G. Lactic acid fermentation of scampi waste in a rotating horizontal bioreactor for chitin recovery. Process Biochemistry 33 (1998) 1- 6.

Jo, G-H.; Park, R-D.; Jung, W-J. Enzymatic production of chitin from crustacean shell waste IN chitin chitosan, oligosaccharides and their Derivatives (2011) Se-Kwon Kim, CRC Press Taylor & Francis Group.

Guinebretière, Rene.; 2006. X-ray diffraction on polycrystalline samples and instrumentation study of the microstructure. Hermes Sciences, Lavoisier, Paris.

Arbia, W. ;Arbia, L.; Adour, L. ;Amrane, A. ;Lounici, H. ;Mameri, N. Kinetic study of biodemineralization and bio-deproteinization of shrimp biowaste for chitin recovery.Algerian Journal of Environmental Science and Technology 3 (2017) 29-36.

Sajomsang, W.; Gonil,P.Preparation and characterization of α-chitin from Cicada sloughs. MaterialsandScienceEngineering C30 (2010).

Mikkelsen, A.; Engelsen, S.B.; Hansen, H.C.B.; Larsen, O.;Skibsted, L.H. Calcium carbonate crystallization in the α-chitin matrix of the shell of pink shrimp, Pandalus borealis, during frozen storage. Journal of Crystal Growth 177 (1997) 125-134.

Zhou, D.; Zhang, L.; Guo, S.; Mechanisms of lead biosorption on cellulose / chitin beads. Water Reseach.39 (2005) 3755-3762.

Mario, F.; Di, P.;Rapana, U.; Tomati, E. G. Chitin and chitosan from Basidiomycetes. Internat.Journal of Biological Macromolecules 43 (2007) 8- 12.

Stawski, D.; Rabiej, S.; Herczynka, L.; Draczynski, Z. Thermogravimetric analysis of chitins of different origin.JournalThermal Calorimetry93 (2008) 2, 489- 494.

Heredia, A. M.; Aguilar-Franco, C.;Magaña, C.; Flores, C.; Piña, R.; Velázquez, T. E.;Schäffer, L.;Bucio, V. A.; Basiuk. Structure and interactions of calcite spherulites with α-chitin in the brown shrimp (Penaeusaztecus) shell. Materials Science and Engineering C27 (2007) 8- 13.

Hosoda, N.; Takashi, K. Thin-film formation of calcium carbonate crystals: Effects of functional groups of matrixpolymers. Chemistry of Materials 13 (2001) 688-693.

Jaworska, M.; Kensuke, S.;Gaudon P.;Guibal,E.Influence of Chitosan Characteristics are polymer properties. I: crystallographic properties. Polymer International 52 (2003) 198-205.

Lavall, R.L.;Odilio, B.G.A.;Campana-Filho, S.P. β-chitin from the pens of Loloigo sp.: Extraction and characterization.BioressourceTechnology 98 (2007) 2465- 2472.

Al Sagheer, F. A. ; Al-Sughayer, M. A. ; Muslim, S. ; Elsabee, M. Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. CarbohydratePolymer 77 (2009) 410-419.


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