Environmental impact Assessment of the L-lysine production process from fermentation of glucose by Corynebacterium glutamicum using Super Pro Designer software

H. Amichi, N. Outili, M. Bencheikh lehocine

Abstract


Abstract: The assessment key factors of any bioprocess sustainability are mainly its interaction towards the environment and the capacity to control any generated pollution, beside its social and economical aspects. These factors have direct impact on the decision-making regarding the choice of the relevant technologies at the early stages of the bioprocess design.

Therefore, in the present study we assessed the environmental impact of a chosen model process, namely for the production L-lysine by fermentation using Corynebacterium glutamicum and glucose as the bacteria and the substrate, respectively.

We used the Super Pro Designer® version 9.0 software to compute mass balances of the entry and exit streams. The results, together with all relevant impact categories, which are classified according to calculated mass indices, were used for environmental assessment to deduce the set environmental parameters, as environmental factors and indices.

In order to optimize the environmental performance of the bioprocess, other indices were also calculated. For instance, the results showed that Water was the most influential relevant component from mass point of view with 20.97 and 22.32 kg/kg P for input and output, respectively. Regarding the fact that Water is an exhaustible natural resource, it is necessary to anticipate its recycling. Therefore, as a second step a part of the water recovered during the purification of the main product was recycled along with L-Lysine wastes.  This led to important mitigations of the environmental impacts of Water and L-Lysine as well to a production increase of the latter.

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