Alteration of biochemical markers of liver and kidney in rats, following exposure to pesticide mixtures
Abstract
The objective of this study was to highlight the effects of various pesticide mixtures on biochemical markers of liver and kidney (total protein, creatinine, cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, serum enzymatic activities of alanine aminotransferase and aspartate aminotransferase) in rats. Three pesticide mixtures commonly used on high consumed fruits and vegetables of Algerian cuisine, were selected. Chlorpyrifos, thiacloprid, spiromesifen, cypermethrin, bromuconazole, abamectin, chlorothalonil, methomyl, spirotetramat, deltamethrin, spirodiclofen, and indoxacarb were administered to male Wistar rats once per day at various doses corresponding to their acceptable daily intake (ADI) value for 120 days.
The study showed that the exposure to pesticide mixtures induce an oxidative stress assessed by an increase of the concentration of thiobarbituric acid reactive substances (TBARS) in liver and kidney tissues reflecting lipoperoxidation content as well as depletion of enzyme activities as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Moreover, the three pesticides mixture induced liver and kidney dysfunctions. These significant changes were more apparent in mixtures corresponding to most diversified diets.
It can be concluded that pesticide mixtures affect adversely and alter biochemical markers of liver and kidney even at minimal doses.
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Abdollahi, M.; Ranjbar, A.; Shadnia, S.; Nikfar, S.; Rezaie, A. Pesticides and oxidative stress: a review. Medical Science Monitor10 (2004) RA141-RA147.
Abolaji, A.O.; Awogbindin, I.O.; Adedara, I.A.; Farombi, E.O. Insecticide chlorpyrifos and fungicide carbendazim; common food contaminants mixture; induce hepatic; renal; and splenic oxidative damage in female rats. Human & Experimental Toxicology 36 (2016) 483-493.
Acker, C.I.; Nogueira, C.W. Chlorpyrifos acute exposure induces hyperglycemia and hyperlipidemia in rats. Chemosphere 89 (2012) 602-608.
Aebi, H. Catalase in vitro. Methods in Enzymology 105 (1984) 121-126.
Akhgari, M.; Abdollahi, M. ; Kebryaeezadeh, A.; Hosseini, R.; Sabzevari, O. Biochemical evidence for free radicalinduced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats. Human & Experimental Toxicology 22 (2003) 205-211.
Al-Attar, A.M.; Elnaggar, M.H.R; Elmalki, E.A. Physiological study on the influence of some plant oils in rats exposed to a sublethal concentration of diazinon. Saudi Journal of Biological Sciences 25 (2018) 786-796.
Bebe, F.N.; Panemangalore, M. Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. Journal of Environmental Science and Health, Part B 38 (2003) 349-363.
Bhardwaj, S.; Srivastava, M.K.; Kapoor, U.; Srivastava L.P. A 90 days oral toxicity of imidacloprid in female rats: Morphological, biochemical and histopathological evaluations. Food and Chemical Toxicology 48 (2010) 1185-1190.
Butterfield, D.A.; Lauderback, C.M. Lipid peroxidation and protein oxidation in Alzheimer’s disease brain: potential causes and consequences involving amyloid beta–peptide–associated free radical oxidative stress. Free Radical Biology and Medicine 32 (2002) 1050-1060.
Curl, C.L.; Fenske, R.A.; Elgethum, K. Organophosphorus pesticide exposure of urban and suburban preschool children with organic and conventional diets. Environmental Health Perspectives 111 (2003) 377-382.
Edelstein, C.L. Biomarkers of Acute Kidney Injury. Advanced Chronic Kidney Diseases 15 (2008) 222–234.
El-Demerdash, F.M. Lipid peroxidation, oxidative stress and acetylcholinesterase in rat brain exposed to organophosphate and pyrethroid insecticides. Food and Chemical Toxicology 49 (2011) 1346-1352.
Ellman, G. Tissue sulphydryl groups. Archives of Biochemistry and Biophysics 82 (1959) 70-73.
Eraslan, G.; Kanbur, M.; Silici, S. Effect of carbaryl on some biochemical changes in rats: The ameliorative effect of bee pollen. Food and Chemical Toxicology 47 (2009) 86-91.
Farag, A.T.; Ewediah, M.H.; El-Okazy, A.M. Reproductive toxicology of acephate in male mice. Reproductive Toxicology 14 (2000) 457-462.
Gomes, J.; Dawodu, A.H.; Lloyd, O.; Revitt, D.M.; Anilal, S.V. Hepatic injury and disturbed amino acid metabolism in mice following prolonged exposure to organophosphorus pesticides. Human & Experimental Toxicology 18 (1999) 33-37.
Halliwell, B.; Chirico, S. Lipid peroxidation: its mechanism, measurement and significance. The American Journal of Clinical Nutrition 57 (1993) 715S-724S.
Hariri, A.T., Moallem, S.A.; Mahmoudi, M.; Memar, B.; Hosseinzadeh, H. Sub–acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranal. Food and Chemical Toxicology 48 (2010) 2803-2808.
Hernández A.F., López O., Rodrigo L., Gil F., Pena G., Serrano, J.L.; Parrón T.; Alvarez, J.C.; Lorente, J.A.; Pla, A. Changes in erythrocyte enzymes in humans long–term exposed to pesticides influence of several markers of individual susceptibility. Toxicology Letters 159 (2005) 13-21.
Hocine, L.; Merzouk, H.; Merzouk, S.A.; Ghorzi, H.; Youbi, M. The effects of alpha-cypermethrin exposure on biochemical and redox parameters in pregnant rats and their newborns. Pesticide Biochemistry and Physiology 134 (2016) 49-54.
Igo, J.O.; Gbadegesin, M.A.; Olugbami, J.O.; Adegoke, A.M.; Odunola, O.A.; Anetor, G.O.; Anetor, J.I. A Common Insecticide Induced-Oxidative Stress in Wistar Rats: Significance for Humans and Implications for Nutritional Modulation of Insecticide Toxicity. Journal of the American College of Nutrition 2 (2020) 1-9.
James, K.A.; Hall, D.A. Groundwater pesticide levels and the association with Parkinson disease. International Journal of Toxicology 34 (2015)266-273.
JEFCA: WHO technical report. Principles governing consumer safety in relation to pesticide residues Geneva: World Health Organization Volume 240 (1962).
http://apps.who.int/iris/bitstream/10665/40536/1/WHO-TRS-240.pdf, last accessed 26/01/2019
Jensen, B.H.; Petersen, A.; Christiansen, S.; Boberg, J.; Axelstad, M., Herrmann, S.S.; M.E.; Hass, U. Probabilistic assessment of the cumulative dietary exposure of the population of Denmark to endocrine disrupting pesticides. Food and Chemical Toxicology 55 (2013) 113-120.
John, S.; Kale, M.; Rathore, N.; Bhatnagar, D. Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes. The Journal of Nutritional Biochemistry 12 (2001) 500-504.
Kehrer, J.P. Free radical as mediator of tissue injury and disease. Critical Reviews in Toxicology 23 (1993) 21-48.
Lasram, M.M.; Annabi, A.B.; El Elj, N.; Selmi, S.; Kamoun, A.; El-Fazaa, S.; Gharbi, N. Metabolic disorders of acute exposure to malathion in adult Wistar rats. Journal of Hazardous Materials 163 (2009) 1052-1055
Lawrence, R.A.; Burk, R.F. Glutathione peroxidase activity in selenuimdeficient rat liver. Biochemical and Biophysical Research Communications 71 (1976) 952-958.
Linnainmaa, K. Sister chromatid exchanges among workers occupationally exposed to phenoxy acid herbicides 2;4–D and MCPA. Teratogenesis Carcinogenesis and Mutagenesis 3 (1983) 269-279.
Maalej, A.; Mahmoudi, A.; Bouallagui, Z.; Fki, I.; Marrekchi, R.; Sayadi, S. Olive phenolic compounds attenuate deltamethrin–induced liver and kidney toxicity through regulating oxidative stress, inflammation and apoptosis. Food and Chemical Toxicology 106 (2017) 455-465.
Matés, J.M.; Pérez-Gómez, C.; Núñez de Castro, I. Antioxidant enzymes and human diseases. Clinical Biochemistry (1999) 32: 595-603.
Mc Cord, J.M. The evolution of free radicals and oxidative stress. The American Journal of the Medical Sciences 108 (2000) 652-659.
Misra, H.P.; Fridovich, I. Superoxide dismutase: "positive" spectrophotometric assays. Analytical Biochemistry (1977) 79: 553-560.
Moisan, F.; Spinosi, J.; Delabre, L.; Gourlet, V.; Mazurie, J.L.; Bénatru, I.; Goldberg, M.; Weisskopf, M.G.; Imbernon, E.; Tzourio, C.; Elbaz, A. Association of Parkinson's disease and its subtypes with agricultural pesticide exposures in men: a case–control study in France. Environmental Health Perspectives 123 (2015) 1123-1129.
Ojha, A.; Yaduvanshi, S.K.; Srivastava, N. Effect of combined exposure of commonly used organophosphate pesticides on lipid peroxidation and antioxidant enzymes in rat tissues. Pesticide Biochemistry and Physiology 99 (2011) 148-156.
Ozer, J.; Ratner, M.; Shaw, M.; Bailey, W.; Schomaker, S. The current state of serum biomarkers of hepatotoxicity. Toxicology 245 (2008) 194-205.
Pothu, U.K.; Thammisetty, A.K.; Nelakuditi, L.K. Evaluation of cholinesterase and lipid profile levels in chronic pesticide exposed persons. Journal of Family Medecine and Primary Care 8 (2019) 2073-2078.
Ranjbar, A.; Pasalar, P.; Abdollahi, M. Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers. Human and Experimental Toxicoogyl 21 (2002) 179-182.
Reffstrup, T.K.; Larsen, J.C.; Meyer, O. Risk assessment of mixtures of pesticides. Current approaches and future strategies. Regulatory toxicology and Pharmacology 56 (2010) 174-192.
Rivero, J.; Luzardo, O.P.; Henríquez–Hernández, L.A.; Machin, R.P.; Pestano, J.; Zumbado, M.; Boada, L.D.; Camacho, M.; Valeron, P.F. In vitro evaluation of oestrogenic/androgenic activity of the serum organochlorine pesticide mixtures previously described in a breast cancer case–control study. Science of Total Environment 537 (2015) 197-202.
Sankar, P.; Telang, A.G.; Manimaran, A. Protective effect of curcumin on cypermethrin–induced oxidative stress in Wistar rats. Experimental and Toxicologic Pathology 64 (2012) 487-493.
Sayeed, I.; Parve, S.; Pandey, S.; Bin-Hafeez, B.; Haque, R.; Raisuddin, S. Oxidative biomarkers of exposure to deltamethrin in fresh water fish, Channa punctatus Bloch. Ecotoxicology and Environmental Safety 56 (2003) 295-301.
Seth, V.; Banerjee, B.D.; Chakravorty, A.K. Lipid peroxidation; free radical scavenging enzymes; and glutathione redox system in blood of rats exposed to propoxur. Pesticide Biochemistry and Physiology 71: (2001) 133-139.
Soltaninejad, K.; Abdollahi, M. Current opinion of the science of organophosphate pesticides and toxic stress: a systematic review. Medical Science Monitor 15 (2009) 75-90.
Tariba Lovaković, B.; Pizent, A.; Kašuba, V.; Kopjar, N.; Micek, V.; Mendaš, G.; Dvoršćak, M.; Mikolić, A.; Milić, M.; Žunec, S.; Lucić Vrdoljak, A.: Želježić, D. Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats. Food and Chemical Toxicology 108 (2017) 93-103.
Tiwari, R.K.; Singh, S.; Pandey, R. Assessment of acute toxicity and biochemical responses to chlorpyrifos, cypermethrin and their combination exposed earthworm, Eudrilus eugeniae. Toxicology Reposrts 6 (2019) 288-297.
Uchiyama, M.; Mihara, M. Determination of malondialdehyde precursors in tissues by thiobarbituric acid test. Analytical Biochemistry 47 (1978) 469–474.
Uzun, F.G.; Demir, F.; Kalender, S.; Bas, H.; Kalander, Y. Protective effect of catechin and quercetin on chlorpyrifos–induced lung toxicity in male rats. Food and Chemical Toxicology 48 (2010) 1714-1720.
Yehia, M.A.; El–Banna, S.G.; Okab, A.B. Diazinone toxicity affects histophysiological and biochemical parameters in rabbits. Experimental and Toxicologic Pathology 59 (2007) 215-225.
Yousef, M.I.; Demerdash, F.M.; Kamei, K.I., et al. Changes in some hematological and biochemical indices of rabbits induced by isoflavones and cypermethrin. Toxicology 189 (2003) 223-234.
Zaahkouk, SAM.; Helal, E.G.E.; Abd-Rabo, T.E.I.; Rashed, S.Z.A. Carbamate toxicity and protective effect of vit. A and vit. E on some biochemical aspects of male albino rats. The Egyptien Journal of Hospital Medicine 1 (2000) 60-77.
Zarkovic, N. 4–Hydroxynonenal as a bioactive marker of pathophysiological processes. Molecular Aspects of Medicine 24 (2003) 281-291.
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